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Sequencher DNA序列分析软件是科学家们*的工具。持续发展和改进**过25年,Sequencher提供了**的功能特征,成千上万的出版物都有相关的应用介绍。新手用户可以以少的时间投资产生结果,而有经验的用户会惊叹于功能的深度和控制。Sequencher自带各种专有算法,可以为Sanger, Next-Gen Sequencing (NGS)和RNA-Seq序列数据生成结果。
FastQC质量控制报告
在开始调整数据之前,先停下来考虑一下,我们的数据到底如何?现在我们可以用FastQC Quality Reports。我们在Sequencher中添加了FastQC程序。从Sequence > Analyses > FastQ Quality Report…启动此功能,您可以得到多达12种不同的度量结果。从‘Per base sequence quality’ 到‘Kmer Content’,从Sequence Duplication Levels’ 到 ‘Overrepresented sequences’,结果给出一个易于理解的交通灯系统图以及更详细的图表。
在组装前,使用报表个人数据集的质量,或随时质量。
RNA-Seq
RNA-Seq实验正在为蛋白质编码转录物的研究带来新的理解和知识,是否从不同时间点或在正常状态和疾病状态之间的正常组织。Sequencher家族新的程序是Cufflinks suite,研究RNA-Seq NGS数据的一系列程序。使用您喜欢的aligner对齐RNA-Seq NGS数据到参考序列中,然后使用SAM或BAM结果文件和参考GTF文件来启动Cufflinks。重点使用Cufflinks, Cuffmerge, Cuffdiff以及终数据以表格和图形展示这四个步骤过程的差异表达。Cufflinks suite命令行程序可以使用我们易于使用的图形界面。对于终控件,您也可以访问所有命令行函数,通过简单的点,然后单击Advanced(Edit)对话框。
Your results are important to you. Whether you work with Sanger Sequencing or Next-Gen Sequencing, you will want to compare your results with publically available data. Sequencher Connections is ideal for a quick search or a more detailed and prolonged analysis across multiple databases and with different parameters.
If you want to restore a set number of bases on either or both ends of your trimmed sequences because your trim was too stringent or you want to improve coverage, Batch Revert Trim Ends lets you do that. With a few clicks, you can restore bases to a few or a few thousand sequences, and get even more control over your sequence trims.
For example, with the click of a button you can convert 90 files, 45 pairs of forward and reverse sequences, into 45 contigs named according to your Patient IDs. A change in your sequence assembly parameters regroups your fragments, so you can assemble the contigs according to Clone ID, Date, Primer, or any other characteristic you record in your sequence names.
,专注,专心是科学软件网的服务宗旨,开发的软件、传递*的技术、提供贴心的服务是我们用实际行动践行的**目标,我们会为此目标而不懈努力。