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The basic unit of most work in GPMAW is a protein sequence. You may choose to enter a sequence manually, load it from a database or directly from the Web (e.g. see B.1/2 and C.1). However, when you work with a sequence on several occasions, you will normally save it to a file on your hard drive. The main advantage to saving sequences locally, in addition to faster access, is that the sequence saved from GPMAW may contain additional information, e.g. modified residues, cross-links, annotations etc. Note that GPMAW sequence libraries may contain multiple sequences. This works in the way that when you save to a file, which already contains one or more sequences, the new one is appended to the file, instead of replacing it.
he Close button closes the sequence window and all daughter windows (i.e. peptide, cleavage, search and graphical windows)
当前的局限
如果修改了带电残留物,则原始pka值仍作为总pi/电荷的一部分进行计算。如果未充电的残余物变为带电的残余物并pka值,则计算“正确”。如果一个界点被修改,它仍然会产生π/电荷。
Are often used to transmit information from daughter windows (i.e. mass search) to the main sequence window.
As they are residue-related, they are always specific to a given sequence.
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