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Species Identification
Using AlleleID®, you can design assays for identifying species and strains from a mix of sequences
Species Identification Assays/Cross Species Assays/Allele Identification Assays
AlleleID® aligns sequences using ClustalW and analyzes conserved and species specific regions. You can then use the program for real time PCR primer design (SYBR® Green primer design included) and dual labeled probe design (TaqMan® probes, TaqMan® MGB probes and molecular beacons). These assays are designed to detect only the strain (strain detection) or species of interest from the mix.
AlleleID® Provides Several Design Options for Designing Taxa Specific/Cross Species Assay
Conserved Probes with Conserved Primers: AlleleID® identifies conserved regions by aligning sequences and then designs a probe within them. This enables identification of any sequence in the set with a single probe. It also designs primer pairs in the conserved regions flanking the probes. As a result, a single primer pair can amplify all the sequences of a group or a taxa.
Species Identification: Minimal probes with Optimal primers
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