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Sequencher DNA序列分析软件是科学家们必备的工具。持续发展和改进超过25年,Sequencher提供了无与伦比的功能特征,成千上万的出版物都有相关的应用介绍。新手用户可以以少的时间投资产生结果,而有经验的用户会惊叹于功能的深度和控制。Sequencher自带各种专有算法,可以为Sanger, Next-Gen Sequencing (NGS)和RNA-Seq序列数据生成结果。
自动化分析
Sequencher批量处理您的DNA序列数据的方式是透明的、用户可定义的以及可恢复的,
Sequencher不会为了自动化而影的科学结论的有效性。Sequencher总是给您序列编辑的终选择。
Sequencher始终保持两个数据副本,编辑的和原始导入的数据。当将恢复到实验数据命令应用到项目中的序列选择或序列中的基础选择时,可以撤消所有或部分编辑内容。按名称组装工具允许您选择片段名称的一部分作为作为共享标识符或“汇编句柄”。Sequencher自动选择和命名重叠群。Sequencher甚至支持正则表达式匹配来设置的ID!
例如,通过点击一个按钮,您可以将90个文件、45对正向和反向序列转换成45个根据患者ID命名的重叠群。序列组装参数的更改会重新组合片段,因此,您可以根据克隆ID、日期、引物或您在序列名称中记录的任何其他特征来组装重叠群。
Clustal
Clustal[1] has been part of the Sequencher family of plugins since version 4.9. It is a widely used multiple-sequence alignment program which works by determining all pairwise alignments on a set of sequences, then constructs a dendrogram grouping the sequences by approximate similarity and then finally performs the alignment using the dendogram as a guide. You can use Clustal to align your sequences directly from the Sequencher project window. Choose from a range of parameters to control the alignment process.
Once the process is complete and MUSCLE has built the alignment, you will see the results as a contig within Sequencher. Use Sequencher’s tools to annotate your alignment or export your alignment and place it into a special phylogenetics program.
Gene Codes has long been an innovator, investing in the R&D to develop powerful features for your DNA sequence analysis. Gene Codes developed the Assemble to Reference Sequence strategy that is widely used to speed up assembly and assign base-numbering systems and features to new data. The variance table was developed in the mid 1990's and became a key element first for forensic sequencing of mtDNA, and then for virtually all of our collaborators.
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